Immunoassay identification ofAspergillus flavus using monoclonal antibodies raised to the whole cell extracts |
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| Authors: | A A G Candlish M S Wibowo J E Smith |
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| Institution: | (1) Department of Bioscience and Biotechnology, University of Strathclyde, 204 George Street, G1 1XW;(2) Department of Pharmacy, Institut Teknologi Bandung, 40132 Bandung, Indonesia |
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| Abstract: | Five separate monoclonal antibodies were produced against whole cell extracts ofAspergillus flavus and ELISA procedures used to characterise the reactivity of the antibodies to various fungal extracts. All five antibodies
were specific to the aflatoxin producing fungi,A, flavus andA. parasiticus, and indicated no cross reactivity with otherAspergillus species, genera of several fungi or with other components which may be found in food samples whereA. flavus may be found. |
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