Zebrafish Genetic Map with 2000 Microsatellite Markers |
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Authors: | Nobuyoshi Shimoda Ela W Knapik John Ziniti Chng Sim Erika Yamada Stacy Kaplan Donald Jackson Frederic de Sauvage Howard Jacob Mark C Fishman |
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Institution: | Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA. |
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Abstract: | The zebrafish is the first vertebrate organism used for large-scale genetic screens seeking genes critical to development. These screens have been quite successful, with more than 1800 recessive mutations discovered that speak to morphogenesis of the vertebrate embryo. The cloning of the mutant genes depends on a dense genetic map. The 2000 markers we present here, using microsatellite (CA) repeats, provides 1.2-cM average resolution. One centimorgan in zebrafish is about 0. 74 megabase, so, for many mutations, these markers are close enough to begin positional cloning by YAC walks. |
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Keywords: | zebrafish linkage map microsatellite repeat polymorphism microsatellite expansion |
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