Folacin C60 derivative exerts a protective activity against oxidative stress-induced apoptosis in rat pheochromocytoma cells |
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| Authors: | Zhen Hu Wenchao Guan Wei Wang Zhou Zhu Yanhong Wang |
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| Institution: | 1. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, China;2. College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China;3. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | In the present study, we describe the synthesis and characterization of a novel folacin C60 derivative. The compound was analyzed by FT-IR, 1H NMR, 13C NMR, LC–MS and elemental analysis. This water soluble fullerene derivative was able to scavenge both superoxide and hydroxyl radical with biocompatibility. Rat pheochromocytoma (PC12) cells treated with hydrogen peroxide underwent cytotoxicity and apoptotic death determined by MTT assay and flow cytometry analysis. As a novel derivative of C60, the folacin C60 derivative self-assembled to form spherical aggregates in H2O. Because the compound was amphiphilic, it could penetrate the cell membrane and play its distinguished role in protecting PC12 cells against hydrogen peroxide-induced cytotoxicity. The results suggest that folacin C60 derivative has the potential to prevent oxidative stress-induced cell death without evident toxicity. |
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