Visualizing antibody-catalyzed retro-aldol-retro-Michael reactions. |
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| Authors: | F Tanaka L Kerwin D Kubitz R A Lerner C F Barbas |
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| Institution: | Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. |
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| Abstract: | We developed a visible detection system for antibody-catalyzed retro-aldol-retro-Michael reactions. Aldolase antibody 38C2 catalyzed the reaction of substrate 1 to provide 6-bromo-2-napthol that forms a visible colored azo dye with diazonium salts. This system has potential for the screening of novel catalysts. |
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