Chondroitin sulphate at the endothelial lumen of pig aorta: Assay by radiolabelling and by X-ray microanalysis |
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Authors: | G Hale H B Streeter A C Hann P Gacesa F S Wusteman |
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Institution: | (1) Department of Biochemistry, University College, P.O. Box 78, CF1 1XL Cardiff |
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Abstract: | Trypsin-releasable glycosaminoglycans from the luminal surface of intact pig aorta were measured following metabolic labelling with35S]sulphate. Chondroitin sulphate was found to be present at a surface density equal to that already established for heparan sulphate (5×1011 chains per cm2). This result was confirmed by X-ray microanalysis of the luminal sulphur content before and after treatment with specific glycosaminoglycan-degrading enzymes. This result implies that approximately half of the luminal surface is occupied by sulphated glycosaminoglycans. |
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Keywords: | chondroitin sulphate endothelium aorta X-ray microanalysis |
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