| Abstract: | A rapid, sensitive, and simple high-performance liquid chromatographic (HPLC) method with an ultraviolet detector (UV) has been developed for the determination of oxaliplatin in the plasma of rabbits and tissues of mice. The sample preparation was carried out by complexation with 0.5?mL of DETC (diethyl-dithiocarbamate) solution and extracted by ether and chloroform. Then, 20?μL of supernatant was injected into the HPLC system with 0.25?mol/L of sodium chloride solution and methanol (30:70 v/v) as the mobile phase at a flow rate of 1.0?mL/min. Separation was performed with a C18 column at 25°C. The peak was detected at 254?nm. The calibration curve was linear (R2?≥?0.9995) in the concentration range of 0.1~200?μg/mL in plasma and tissues. The intra- and interday variation coefficients were not more than 2.61 and 3.83%, respectively. The limit of detection was 20?ng/mL. The mean recoveries of oxaliplatin were ranged from 97.83 to 104.17% in plasma and tissues. The present method has been successfully applied to the pharmacokinetic study of oxaliplatin liposome in mice and rabbits. |
