野牛草幼穗愈伤组织的诱导及植株再生

以野牛草Buchloe dactyloides(Nutt．)Engelm．]幼穗为外植体,建立了愈伤组织诱导、继代培养和植株再生体系。结果表明,雌穗比雄穗难以脱分化形成愈伤组织;小于8mm雄幼穗在2mg／L2,4-D培养基上的愈伤组织诱导率为80．0％～86．8％;添加10mg／L AgNO3对愈伤组织诱导率影响不明显,但可改善愈伤组织质量。2mg／L 2,4-D结合0．1mg／L 6-BA的培养基有利于愈伤组织的继代培养;继代超过3次、继代间隔超过3周,愈伤组织分化能力明显下降。雄穗愈伤组织在含1．0mg／L 6-BA培养基上,弱光条件下分化出芽的频率较高,达31．8％～35．0％;附加3％麦芽糖既可减轻褐化程度,又利于丛生芽的分化。分化苗在1/2MS 0．3mg／L IBA培养基上的生根率为62．5％。

Callus Induction from Immature Inflorescence and Plant Regeneration of Buffalograss

The immature embryo of Actinidia arguta var. purpurea were cultured on three different media to induce callus. The results show the callus was obtained from basic MS media with the combination of 0.2 mg/L ZT and 0.5 mg/L GA_3. High callus induction frequency was obtained from MS media supplemented with 7% surgar, 600 mg/L CH and 400 mg/L Gln. The highest rate of regeneration was found under the MS media supplemented with 0.5 mg/L 6-BA, 0.05 mg/L NAA and 0.5 mg/L GA_3.