| 不同浓度有机物对雷公藤不定根生长和次生代谢产物含量的影响 |
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| 引用本文： | 李琰,崔蕾,雷嘉敏,李群,张兴.不同浓度有机物对雷公藤不定根生长和次生代谢产物含量的影响[J].武汉植物学研究,2014(2):174-180. |
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| 作者姓名： | 李琰 崔蕾 雷嘉敏 李群 张兴 |
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| 作者单位： | [1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学无公害农药研究服务中心、陕西省生物农药工程技术研究中心,陕西杨凌712100 |
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| 基金项目： | 公益性行业（农业）科研专项（200903052）;陕西省自然科学基金（2011JM3006）. |
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| 摘 要： | 以NT为基本培养基,雷公藤(Tripterygium wilfordii)不定根为材料,研究了肌醇、VB1、烟酸、VB6、甘氨酸、叶酸、生物素等有机物质对雷公藤不定根生长及其次生代谢产物雷公藤甲素和总生物碱含量的影响。结果表明:NT培养基中原有浓度的肌醇、VB1含量即可使雷公藤不定根生长量、雷公藤甲素含量、总生物碱含量及产量达到最大值。在添加的其他有机物中,添加1 mg/L烟酸、1 mg/L VB6和5 mg/L甘氨酸适合不定根的生长;添加0.5 mg/L烟酸、0.5 mg/L生物素、1 mg/L VB6、1 mg/L甘氨酸和1 mg/L叶酸适合雷公藤甲素的积累;添加0.5 mg/L甘氨酸、1 mg/L VB6、1 mg/L叶酸和1 mg/L生物素则适合不定根中雷公藤总生物碱的合成。
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| 关 键 词： | 雷公藤 不定根 组织培养 有机物 次生代谢产物 |
Effects of Different Concentrations of Organic Affixture on the Growth and Secondary Metabolites Contents in Adventitious Roots of Tripterygium wilfordii |
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| LI Yan,CUI Lei,LEI Jia-Min,LI Qun,ZHANG Xing.Effects of Different Concentrations of Organic Affixture on the Growth and Secondary Metabolites Contents in Adventitious Roots of Tripterygium wilfordii[J].Journal of Wuhan Botanical Research,2014(2):174-180. |
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| Authors: | LI Yan CUI Lei LEI Jia-Min LI Qun ZHANG Xing |
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| Institution: | 1. College of Life Science, Northwest A＆F University, Yangling, Shaanxi 712100, China 2. Research and Development Center of Biorational Pesticide Northwest A ＆ F University / Shaanxi Province Technology and Engineering Center of Biopesticide, Yangling, Shaanxi 712100, China) |
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| Abstract: | Taking NT as the basic medium, the effect of different concentrations of organic affixture on the growth of adventitious root and accumulation of secondary metabolites were studied in Tripterygium wilfordii. The results showed that the optimal NT medium component of inositol and VB1 was the best standard not only for the growth of adventitious root, but also for the accumulation of total alkaloid and triptolide. The adventitious root increment was significantly improved by other organic affixtures including 1 mg/L niacin, 1 mg/L VB6, and 5 mg/L glycine. Total alkaloid content was significantly improved by 0.5 mg/L niacin and 0.5 mg/L biotin, 1 mg/L VB6, 1 mg/L glycine and 1 mg/L folate. The test of 0.5 mg/L glycine, 1 mg/L VB6, 1 mg/L folate and 1 mg/L biotin were the highest for triptolide content. |
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| Keywords: | Tripterygium wilfordii Adventitious root Tissue culture Organic affixture Se-condary metabolites |
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