藏药纤毛婆婆纳的愈伤组织诱导和快速繁殖研究

以纤毛婆婆纳（Veronica ciliateFisch.）无菌苗的顶芽作为外植体,在不同激素和浓度组合的MS培养基上进行愈伤组织诱导和快速繁殖的研究。结果表明,愈伤组织诱导的最佳培养基为：MS＋6-BA 0.5 mg/L＋NAA1.0 mg/L,诱导率达到95%;顶芽在MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L增殖培养基中增殖效果最佳,增殖系数高达5.4;丛生芽在1/2 MS＋IBA 0.05 mg/L生根培养基生根效果最好,不仅根的质量好,而且生根率也达到95%;此再生苗的移栽成活率也最高,在适宜条件下可达40%。

Callus Induction and Rapid Propagation Systems from a Tibetan Medicine Plant, Veronica ciliate Fisch.

To induce callus and establish rapid propagation systems for Veronica ciliate Fisch.,terminal buds from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA.Results showed that the optimum medium for callus inducement was MS+6-BA 0.5 mg/L+NAA 1.0 mg/L,with the callus induction rate of 95%.The best medium for multiplication was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,with the multiplication coefficient reaching 5.4.The optimum root medium for multiple shoot clumps...