尖叶拟船叶藓原丝体发育特征研究

将尖叶拟船叶藓Dolichomitriopsis diversiformis(Mitt.)Nog.]孢子接种于Knop培养基上,置于恒温培养箱中培养,在光学显微镜下对其原丝体(protonema)发育特征进行了详细观察和记录。结果表明:孢子第2天就开始萌发,第6天时其萌发率达90%以上;原丝体系统由绿丝体(chloronema)和轴丝体(caulonema)构成,假根(rhizoides)产生于芽体基部,由轴丝体退化而成;配子枝原始细胞产生于绿丝体分枝的基部或轴丝体上的斜壁细胞;配子枝(game tophore)形成后其上各部位都可形成假根;孢子萌发类型为真藓型(Bryum-type)。

Study on the Characteristics of Protonema Development in Dolichomitriopsis diversiformis

Spores of Dolichomitriopsis diversiformis were planted on Knop medium and cultured in incubator,and the process of spore germination and protonema growth were illustrated and microphotographs were taken.The results are as follows:spores began to germinate in the 2nd day and over 90% of them germinated in the 6th day.Protonema system was composed of the chloronema and caulonema.The rhizoides were formed from the bud base and produced from the caulonema which were degenerated.Gametophore primordium occurred at the branch base of the chloronema or at the caulonema cells which have cross walls that are oblique to the filament axis.Rhizoides were produced from nearly any part of the gametophore.The germination type of its spore was bryum type.