石杉科植物石杉总碱含量的测定及基于紫外光谱的除趋势对应分析

对来自浙江、福建境内石杉科植物蛇足石杉（Huperzia serrata）、柳叶马尾杉（Phlegmariurus cryptomerianus）10个不同地理居群的石杉总碱含量进行了测定．并基于紫外光谱，对它们的表型分化进行了除趋势对应分析。结果表明：①石杉科这两种植物叶片的石杉总碱含量普遍高于茎中的含量；②来自浙江磐安的蛇足石杉居群的叶、茎石杉总碱含量都明显高于其它居群。可以选择该居群作为栽培蛇足石杉的种源；③DCA排序发现，产自磐安的蛇足石杉样品，特别是叶的提取液，其紫外光谱明显不同于其它样品．表明紫外光谱法能够用于鉴别石杉总碱含量较高的蛇足石杉药材。

Content Determination of Assemble Alkaloids of Huperzine in Huperziaceae and Dedentred Correspondence Analysis Based on Their UV Spectra

The content of assemble alkaloids of huperzine in 10 different geographical populations of Huperzia serrata and Phlegmariurus cryptomerianus from Zhejiang and Fujian Province was determined,and their phenetic differentiation in chemical composition was studied using the method of dedentred correspondence analysis(DCA) based on their UV spectra.The results showed that:(1)The content of assemble alkaloids of huperzine in leaves are higher than those in stems of both H.serrata and P.cryptomerianus;(2)the content of assemble alkaloids of huperzine in H.serrata in the population from Pan'an is the highest in either leaves or stems,compared with those in other nine populations,thus this population could be considered as the best choice for the cultivation;(3)DCA ordination on their UV spectra showed that the UV spectra of the leaf extracting solution form H.serrata of Pan'an was distinctly different from others,revealing the UV spectra could be applied to identify the sample with higher content of assemble alkaloids of huperzine.