三叶木通果实对小鼠急性毒性的初步研究

以三叶木通（Akebia trifoliata）果实为试材，选取体重20～23g的昆明种小鼠作为试验对象，分别研究果皮、果肉、种子对小鼠的急性毒性效应。结果表明，三叶木通果实的果皮、果肉对小鼠无毒，果皮滤液的最大耐受量大于50g／k，果肉的最大耐受量大于100g／kg。而种子与蒸馏水1：1的混悬液却对小鼠有毒，LD50=12．83g／kg。灌胃后，绝大多数小鼠5～10min内自发活动减少，呈嗜睡状态，1～2h死亡，死亡前少数有轻度惊厥（后肢向后伸），呼吸先停，随后心跳停止；未死亡小鼠中毒症状持续约3—4h，随后小鼠的饮食、活动恢复正常。

The Primary Study of Acute Toxicity Induced by Fruits of Akebia trifoliate on Mice

The fruits of the cultivated Akebia trifoliata and the mice (20-30 g) of species 'Kunming' were taken as experimental materials.The acute toxicity of pericarp,flesh and seed on these mice were tested respectively.The results indicated that the pericarp and flesh of Akebia trifoliata had no toxicity on these mice.The maximum tolerant dosage for the filtered percarp mixture was 50 g/kg to mice,and the maximum tolerant dosage for the flesh was 100 g/kg to mice.However,the diluted suspended mixture of seeds and distilled water at the ratio of 1 ∶1 was toxic to the mice(LD_(50)=12.83 g/kg).Spontaneous activities of most mice reduced and showed somnolent after the suspended seed mixture was given intragastrically for 5 to 10 minutes,and then died within 1 or 2 hours.A few mice showed light convulsions (hind stretched backwards) before death.Their respiration stopped first and the heart-throb ended subsequently.The toxic symptom of the survived mice continued for 3 or 4 hours and the mice could recover to normal diet and action later.