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春兰菌根的显微结构及菌根真菌的分离
引用本文:余知和,曾昭清,张明涛.春兰菌根的显微结构及菌根真菌的分离[J].武汉植物学研究,2009,27(3):332-335.
作者姓名:余知和  曾昭清  张明涛
作者单位:长江大学生命科学学院,湖北荆州,434025
基金项目:湖北省教育厅青年项目 
摘    要:以盆栽春兰和野生春兰的根为材料,通过切片观察菌根真菌的分布特点和菌根的显微结构。同时采用组织分离方法从盆栽和野生春兰根分别分离得到6个和15个真菌菌株,分离菌株的出现频率分别为16.7%~50%和6.7%~73.3%,并由此判断CGP-3为盆栽春兰菌根的优势菌种,CGW-3为野生春兰菌根的优势菌种。经形态学观察将其初步分为6个分类单元,其中CGP-2和CGW-2分别具有拟日本曲霉(Aspergillus japonicus-like)和拟木霉属(Trichoderma sp.-like)的形态特征。此外,对兰科棺物与内生真菌间的专一件讲行了讨论。

关 键 词:春兰  菌根真菌  分离  菌根显微结构

Mycorrhizal Microstructure of Cymbidium georingii and Its Mycorrhizal Fungi Isolation and Identification
YU Zhi-He,ZENG Zhao-Qing,ZHANG Ming-Tao.Mycorrhizal Microstructure of Cymbidium georingii and Its Mycorrhizal Fungi Isolation and Identification[J].Journal of Wuhan Botanical Research,2009,27(3):332-335.
Authors:YU Zhi-He  ZENG Zhao-Qing  ZHANG Ming-Tao
Institution:( College of Life Sciences, Yangtze University, Jingzhou, Hubei 434025, China)
Abstract:The distribution patterns of mycorrhizal fungi and the microstructure of mycorrhizae in pot cultured and wild Cymbidium georingii were investigated and observed. Six strains of mycorrhizal fungi were isolated and obtained from the mycorrhizae in pot cultured C.georingii and fifteen from the wild ones, which their frequency was 16.7% - 50% and 6.7% - 73.3% , respectively. According to this data of frequency, the strain CGP-3 would be dominant one of pot cultured and CGW-3 would be predominance in wild C. georingii. On the basis of morphological observation all of 21 strains isolated were identified into six taxa, two of them were similar morphological appearances as AspergiUus japonicus-like and Trichoderma sp. -like. By the way, the specificity between the endomycorrhizal fungi and arethusa was also discussed briefly.
Keywords:Cymbidium georingii  Mycorrhizal fungi  Isolation  Mycorrhizal microstructure
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