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| 红莲型杂交稻(红莲2号)及其骨干亲本的RAPD分析与鉴定 | |
| 引用本文： | 段世华,毛加宁,朱英国.红莲型杂交稻(红莲2号)及其骨干亲本的RAPD分析与鉴定[J].武汉植物学研究,2002,20(3):171-176. |
| 作者姓名： | 段世华 毛加宁 朱英国 |
| 作者单位： | 1. 武汉大学植物发育生物学教育部重点实验室,武汉大学遗传研究所,武汉,430072;江西井冈山师范学院生物系,江西吉安,343009 2. 四川乐山师范学院生物系,四川乐山,614000 3. 武汉大学植物发育生物学教育部重点实验室,武汉大学遗传研究所,武汉,430072 |
| 基金项目： | 国家转基因专项基金资助项目 (J99-A-0 0 2 |
| 摘 要： | 利用RAPD技术，从248个随机寡核苷酸引物（10－mer）中筛出18个引物对红莲型杂交稻组合红莲2号及其亲本（T－07A、T－07B、YD6－05），另6个红莲型胞质不育系的骨干恢复和汕优63及其亲本共14份水稻材料进行分析。共检测到173个多态性标记。聚类分析结果表明：不育系与保持系间因核背景相似，遗传差异很小；杂种（F1）的基因型更倾向于恢复系；恢复系与保持系间遗传距离的相对较大，但各恢复系之间的遗传距离较小。利用这些标记能有效地地区交组合中不育系，保持系、恢复系和杂种（F1）。 |
| 关 键 词： | 杂交水稻 RAPD 骨干亲本 遗传分析 鉴定 |
| 文章编号： | 1000-470(2002)03-0171-06 |
| 收稿时间： | 2001-9-17 |
| 修稿时间： | 2001-11-27 |
Genetics Analysis and Identification of Hybrid Rice HL-Type (Honglian-2) and Their Backbone Parental with RAPD Markers |
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| DUAN Shi-Hua,MAO Jia-Ning,ZHU Ying-Guo.Genetics Analysis and Identification of Hybrid Rice HL-Type (Honglian-2) and Their Backbone Parental with RAPD Markers[J].Journal of Wuhan Botanical Research,2002,20(3):171-176. | |
| Authors: | DUAN Shi-Hua MAO Jia-Ning ZHU Ying-Guo |
| Institution: | 1. The Key Laboratory of MOE for Plant Developmental Biology, Institute of Genetics, Wuhan University, Wuhan 430072, China;2. Department of Biology, Jinggangshan Normal College, Ji'an, Jiangxi 343009, China;3. Department of Biology, Leshan Normal College, Leshan, Sichuan 614000, China |
| Abstract: | A total of 248 arbitrary 10 mer oligonucleotide primers were screened using RAPD(random amplified polymorphic DNA) technique on the genome DNAs of 14 rice materials which included HL type hybrid rice (Honglian 2) and their parental(T 07A,T 07B,YD6 05),six other backbone restore lines of CMS Honglian type,Shanyou 63 and their parental.Eighteen primers produced 173 polymorphic fragments.The results from cluster analysis showed:There was little genetic diversity between sterile lines and maintainer lines because of their similar nuclear background.The genome type of hybrid rice was inclined to restore lines.There was large genetic variation between restorer lines and sterile lines,but the genetic distance was small among restorer lines.Using this RAPD markers,the hybrid rice combinations (sterile line,maintainer line,resto rer line and hybrid rice(F 1)) could be effectively identified. |
| Keywords: | Hybrid rice RAPD Backbone parental Genetics analysis Identification |
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