耐盐突变体小麦后代耐盐稳定性分析研究

以卫星搭载小麦种子为原始材料，利用其幼穗、幼胚诱导的愈伤组织进行耐盐突变体的筛选，对耐盐愈伤组织再生植株后代进行耐盐稳定性生理生化特性分析。结果表明：(1)耐盐系后代在土壤高盐浓度条件下，游离脯氨酸含量稳定增加，且高于对照系；(2)耐盐系再生植株后代保持较高的K^ ／Na^ 比；(3)与对照相比，种子醇溶蛋白电泳带谱中的b2，b3，b5，b7带为耐盐系所特有，b8带消失；(4)耐盐系再生植株后代可溶蛋白电泳带为26条，而对照系为23条蛋白带。其中98kD、75kD、52kD、49kD和32kD为耐盐系的特有蛋白带。而38kD和35kD蛋白带为对照系所特有。

Study on Physiological and Biochemical Characterization in Progenies of Selection of NaCl-tolerant Wheat Mutant by in vitro

Salt-tolerant callus was selected from immature embryo and young inflorescence segments of wheat ( Triticum aestivum ) which seeds were carried by spacecraft,and the salt tolerant stability of their progenies were studied on physiological and biochemical characterization.The results showed that:(1) The relative content of free proline in salt-selected wheat plantlet's progencies was higher than that in unselected ones at higher NaCl concentration soils;(2) The salt-selected lines had higher K~+/Na~+ ratio;(3) Four additional specific electrophoretic bands of gliadin (b2,b3,b5 and b7) were detected and one band (b8) disappeared in F_4 seeds from salt-selected lines;(4) 26 electrophoretic bands of soluble protein were observed in F_4 plants from salt-selected lines,whereas only 23 bands in control lines.Of which 98 kD,75 kD,52 kD,49 kD and (32 kD) bands were specific in salt-tolerant line,38 kD and 35 kD protein bands only appeared in (control) lines.