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镉胁迫对丹参生长及有效成分积累的影响研究
引用本文:张鑫,李昆伟,陈康健,梁健,崔浪军.镉胁迫对丹参生长及有效成分积累的影响研究[J].武汉植物学研究,2013(6):583-589.
作者姓名:张鑫  李昆伟  陈康健  梁健  崔浪军
作者单位:[1]药用植物资源与天然药物化学教育部重点实验室/陕西师范大学生命科学学院,西安710062 [2]勉县第一中学,陕西勉县724200
基金项目:基金项目:科技部科技惠民计划项目(2012GS610102);教育部高等学校博士学科点专项科研基金(200807181002);陕西师范大学优秀科技预研项目(200902003);上海辰山植物园科研专项课题(G102404).
摘    要:采用盆栽试验方法,研究了镉(Cd2+)对丹参(Salvia miltiorrhiza Bunge)的生长及其有效成分积累的影响。结果表明,镉胁迫下丹参的生长受到了显著抑制,体内Cd2+残留量、可溶性蛋白含量和膜脂过氧化程度显著增加,叶绿素含量降低。与对照相比,镉胁迫下丹参叶片水溶性酚酸类化合物咖啡酸和迷迭香酸的含量显著降低(P〈0.05),原儿茶酸含量增加,丹参素、原儿茶醛和丹酚酸B的含量也降低,但变化均不显著(P〉0.05)。而根系中这6种酚酸类化合物含量均降低,其中迷迭香酸的含量变化极显著(P〈0.01)。镉胁迫下丹参根系脂溶性丹参酮类化合物二氢丹参酮、丹参酮I和隐丹参酮含量均显著降低,而丹参酮lIA含量变化不显著。丹参叶片中水溶性酚酸类化合物合成关键酶苯丙氨酸解氨酶(PAL)和酪氨酸氨基转移酶(TAT)的活性显著降低,而肉桂酸4一羟化酶(C4H)和4一香豆酸CoA连接酶(4CL)的活性显著升高。这些结果均说明镉胁迫可以降低丹参的产量和质量。

关 键 词:镉胁迫  丹参  生长状况  有效成分

Effects of Cadmium Stress on Seedlings Growth and Active Ingredients in Salvia miltiorrhiza
ZHANG Xin,LI Kun-Wei,CHEN Kang-Jian,LIANG Jian,CUI Lang-Jun.Effects of Cadmium Stress on Seedlings Growth and Active Ingredients in Salvia miltiorrhiza[J].Journal of Wuhan Botanical Research,2013(6):583-589.
Authors:ZHANG Xin  LI Kun-Wei  CHEN Kang-Jian  LIANG Jian  CUI Lang-Jun
Institution:1. Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, College of Sciences, Shaanxi Normal University, Xi'an 710062 China 2. No. 1 Middle School in Mianxian, Mianxian, Shaanxi 724200, China)
Abstract:Effects of cadmium stress on seedlings growth and active ingredients in Salvia miltiorrhiza were investigated. Results showed that cadmium stress inhibited seedlings growth and increased Cd2+ accumulation in both soil and seedlings. Moreover, both soluble protein content and membrane lipid peroxidation degree increased, while photosynthetic pigments content decreased under stress conditions. Compared with the control, in stressed S. miltiorrhiza leaves, the accumulation of six phenolic acid compounds showed different variant characteristics. Both caffeic acid and rosmarinic acid accumulation decreased significantly (p〈0.05), while the content of protocatechuic acid increased (p〉 0.05), and the levels of tanshinol, protocatechuic aldehyde and salvianolic acid B decreased (p〉 0.05). However, the accumulations of the phenolic acids components and four tanshinone ingredients all decreased in stressed roots, and the variances of rosmarinic acid, dihydrotanshinone, tanshinone I and cryptotanshinone were significant (p〈0. 05). Moreover, in the leaves of the stressed plant, the activities of PAL and TAT decreased significantly, but C4H and 4CL content increased significantly. These results showed that cadmium stress could reduce the production and quality of S. miltiorrhiza.
Keywords:Cadmium stress  Salvia miltiorrhiza Bunge  Growth condition  Active ingredients
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