| RAPD双引物在构建红麻连锁图谱中的应用研究 |
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| 引用本文： | 徐建堂,陈美霞,谢曾荣,祁建民,方平平,陶爱芬,林荔辉.RAPD双引物在构建红麻连锁图谱中的应用研究[J].武汉植物学研究,2010,28(6):666-672. |
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| 作者姓名： | 徐建堂 陈美霞 谢曾荣 祁建民 方平平 陶爱芬 林荔辉 |
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| 作者单位： | 福建农林大学作物遗传育种与综合利用省部共建教育部重点实验室,福州 350002 |
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| 基金项目： | 国家自然科学基金资助项目，国家948项目，国家科技支撑项目 |
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| 摘 要： | 以阿联红麻（Alian Kenaf）与福红992（Fuhong992）杂交产生的F2代作图群体为研究材料,分别应用RAPD单引物和双引物进行多态性条带扩增,并进行扩增效果的比较研究,以期为作图群体构建红麻遗传连锁图谱奠定基础。结果表明,RAPD双引物比单引物扩增出更多的多态性条带,提高了引物的利用率和多态性条带的扩增效率。
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| 关 键 词： | RAPD双引物 红麻 遗传连锁图谱 |
Applied Research on Construction of a Genetic Map of Kenaf Using Two-primer RAPD Combinations |
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| XU Jian-Tang,CHEN Mei-Xia,XIE Zeng-Rong,QI Jian-Min,FANG Ping-Ping,TAO Ai-Fen,LIN Li-Hui.Applied Research on Construction of a Genetic Map of Kenaf Using Two-primer RAPD Combinations[J].Journal of Wuhan Botanical Research,2010,28(6):666-672. |
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| Authors: | XU Jian-Tang CHEN Mei-Xia XIE Zeng-Rong QI Jian-Min FANG Ping-Ping TAO Ai-Fen LIN Li-Hui |
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| Institution: | (Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops(Fujian Agriculture and Forestry University),Fuzhou 350002,China) |
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| Abstract: | RAPD and two-primer RAPD combinations were used to screen and compare for polymorphisms between Alain and Fuhong992,providing the foundation for constructing a genetic map of kenaf.The results showed that RAPD was a potential tool for constructing a genetic linkage map of kenaf.PCR reactions with two different primers tended to produce different amplification products than the single primer,so using two different primers was a convenient and economical way of improving polymorphism. |
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| Keywords: | Two-primer RAPD combinations Kenaf Genetic linkage map |
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