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小麦不同生理状态的幼穗和幼胚盾片与诱导分化能力关系的研究
引用本文:何勇刚,林刚,刘曼西,何光源.小麦不同生理状态的幼穗和幼胚盾片与诱导分化能力关系的研究[J].武汉植物学研究,2001,19(5):363-368.
作者姓名:何勇刚  林刚  刘曼西  何光源
作者单位:华中科技大学生命科学与技术学院中英HUST-IACR作物基因工程和基因组学联合实验室,
基金项目:国家“九五”攻关项目资助(编号:96-002-02-24).)
摘    要:用植物组织培养的方法,研究了冬小麦品种鄂恩1号和品系鄂55072不同生理状态的幼穗和幼胚盾片与诱导分化的关系。结果表明,长度在0.4-2.0cm间的幼穗和直径在0.4-1.5mm间的盾片随生理状态不同,其诱导和分化频率有明显的差异,幼嫩的材料再生频率较高。经SAS统计分析,发现0.5-1.0cm长的幼穗和直径为0.4-1.1mm的盾片是处于诱导分化的最佳生理时期的实验材料,平均每个胚性愈伤组织的植株再生分别达到3.17和5.63株。通过比较幼穗和幼胚盾片的植物组织培养结果,发现幼胚盾片比幼穗愈伤组织出现早,生长快,植株再生绿苗率高,这表明小麦幼胚盾片是较好的植物组织培养的材料。

关 键 词:小麦  幼穗  幼胚盾片  生理状态  组织培养
文章编号:1000-470(2001)05-0363-06
收稿时间:2001-1-4
修稿时间:2001-6-1

The Study of Relation between Differentiation Ability and Different Physiological States of Infloresence and Scutellum from Bread Wheat (Triticum aestivum L.)
HE Yong-Gang,LIN Gang,LIU Man-Xi,HE Guang-Yuan.The Study of Relation between Differentiation Ability and Different Physiological States of Infloresence and Scutellum from Bread Wheat (Triticum aestivum L.)[J].Journal of Wuhan Botanical Research,2001,19(5):363-368.
Authors:HE Yong-Gang  LIN Gang  LIU Man-Xi  HE Guang-Yuan
Institution:China-UKHUST-IACR Crop Genetic engineering and Genomics Joint Laboratory, HUST, IACR, Wuhan 430074, China
Abstract:In order to choose the suitable target source and developmental stage of wheat explants for establishment of high frequency of plant regeneration system,inflorescence and scutellum explants were cultured in vitro conditions to study the effects of the explants physiological state on the frequencies of callus induction and plant regeneration.Three size classes of inflorescence(1.3~2.0 cm,0.8~1.2 cm,0.4~0.7 cm in length) and immature embryo scutellum(1.2~1.5 mm,0.8~1.1 mm,0.4~0.7 mm in diameter) were used in this study from two winter wheat genotypes En1 and E55072.According to statistical analysis,the results indicated that the explants showed significantly differences in terms of callus induction and plant regeneration in in vitro culture from explants with different size classes.For inflorescence,explants with 0.5~ 1.0 cm in length give better response,from which per embryogenic callus produced 3.17 plantlets.For scutellum,explants with 0.4~1.1 mm in diameter gave better response,from which per embryogenic callus generated 5.63 plantlets.
Keywords:Triticum aestivum L    Inflorescence  Scutellum  Physiological statue of explants  In vitro culture
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