马唐体细胞胚胎发生过程中生理变化的研究

本文通过对马唐体细胞胚胎发生过程中生理变化的研究后发现,球形胚游离氨基酸种类最少、浓度最低;其过氧化物酶、酯酶和淀粉酶同工酶活性较高、种类较多;其可溶性蛋白质相对浓度最高,并且出现至少两种新带。这说明球形胚已开始分子水平的分化;在这一时期合成的蛋白质(酶),对于马唐体细胞胚胎发生中胚体细胞水平的分化有着重要作用。

STUDIES ON PHYSIOLOGICAL CHANGES DURING SOMATIC EMBRYOGENESIS OF DIGITARIA SANGUINALIS(L.) SCOP.

A somatic embryogenesis experimental system at a high frequency in Digitaria sanguinalis (L.) Scop.was established Using this system, the role of macromolecular synthesis during somatic embryogenesis was investigated. The soluble protein content was determined, and the internal free amino acids were chromatographed, and soluble protein, peroxidase, esterase, and diastase isoenzymes were identified in the extracts of somatic embryoids at different stages. The results obtained show that the content is the highest, but the size of the free amino acid pool which has only seven amino acids is the smallest at globule stage. A protein band(Rf=0.34), three peroxidase isoenzyms(Rf=0.56; 0.59; 0.61) and a diastase isoenzym(Rf=0.57)appeared specifically at globule stage as compared with the other stages. This shows that the globule stage is the key stage of somatic embryogenesis of Digitaria sanguinalis (L.) Scop., and it is possible to use these isoenzymsas molecular marker of somatic embryogenesis.