拟南芥AtDAD1 超量表达植株对H2O2抗性的研究

构建拟南芥AtDAD1超量表达载体，以农杆菌介导的方法转化拟南芥哥伦比亚生态型，比较AtDAD1超量表达植株和野生型植株表现型的差异，以及两者对H2O2抗性的不同。实验显示，AtDAD1转基因拟南芥生长较野生型拟南芥更为强壮，对高浓度H2O2有较强的耐受力。测定两者糖含量，发现AtDAD1转基因拟南芥叶片糖的含量明显高于野生型拟南芥叶片。以上结果表明，AtDAD1基因可能参与植物生长发育，并可能在拟南芥抵抗凋亡的过程中发挥重要的作用。

The Role of Arabidopsis AtDAD1 in the Tolerance to Hydrogen Peroxide Stress

In this research,the differences between wild-type Arabidopsis thaliana and the AtDAD1 overexpression type in their tolerance to high dosages of H_2O_2 were compared.The diversity on their phenotype from the germination to ripeness was also observed.The results demonstrated that the overexpression of AtDAD1 conferred the transgenic plant more resistant to H_2O_2 stress.It is also be found that the sugar content in leaves of transgenic plant is much higher than that in wild-type Arabidopsis leaves.Since many lines of evidence have proved that H_2O_2 is a factor leading to programmed cell death (PCD) and sugar is important for leaf senescence and cell death,it was inferred that AtDAD1 maybe have some effect on Arabidopsis resistance to PCD.