猕猴桃高频直接再生体系的建立

为了建立猕猴桃高频再生体系,以MS为基本培养基,猕猴桃(Actinidia deliciosaQinmei)茎及叶片为外植体,研究了2,4-D、6-BA和NAA在美味猕猴桃愈伤组织形成及分化过程中的作用。方差分析结果表明,6-BA能够显著促进愈伤组织形成,6-BA和NAA可以显著促进愈伤组织形成和分化,而2,4-D抑制愈伤组织形成。附加2.0 mg/L 6-BA、1.0 mg/L NAA和600 mg/L水解酪蛋白的MS培养基是茎段培养的最佳培养基,在该培养基上,以再生的无菌苗为起始材料,一个月时叶圆盘的直接再生频率达到100%,平均每个叶圆盘产生9.33个芽,其中23.21%芽高度超过0.5 cm。

Establishment of High Frequency and Direct Regeneration System of Kiwifruit (Actinidia deliciosa Qinmei)

In order to establish a high frequency regeneration system of kiwifruit,effects of 2,4-D,6-BA and NAA on callus induction and differentiation of stem and leaf explants of Actinidia deliciosa Qinmei were studied with MS as basic medium.The results revealed that callus could be stimulated significantly by 6-BA,and its differentiation could be improved by 6-BA in combination with NAA.In contrast,2,4-D had an negative effect on callus induction.MS medium supplemented with 2.0 mg/L 6-BA and 1.0 mg/L NAA was optimal for shoot regeneration of stem cuttings and leaf discs.On the optimal medium,the leaf discs from the regenerated sterile plants could produce secondary shoot directly and shoot regeneration rate reached 100%.Each leaf disc produced 9.33 shoots on average,and 23.21% of them exceed 0.5 cm in height.