云南紫稻细胞质雄性不育系花药发育过程中Ca2+的分布特点

运用焦锑酸钾沉淀法研究了云南紫稻细胞质雄性不育系和保持系花药在发育过程中Ca^2 的分布特点。结果表明，保持系的花粉母细胞和小孢子的胞质内部基本无Ca^2 的沉淀，后期花粉外壁出现Ca^2 的沉淀；保持系早期的绒毡层细胞形态正常，胞内有少量Ca^2 沉淀，后期绒毡层细胞开始凋亡，胞质凝集，胞内出现大量Ca^2 的颗粒。不育系花粉母细胞在减数分裂时期败育，胞质液泡化，内部出现大量Ca^2 的沉淀；不育系绒毡层细胞形态正常，胞内无Ca^2 的沉淀。绒毡层与花粉母细胞、小孢子之间出现大量Ca^2 颗粒。探讨了不育系花药花粉母细胞中以及与绒毡层细胞之间Ca^2 的异常积累与雄性不育的关系。

The Calcium Distribution in the Anther of Cytoplasmic Male Sterile Line of Yunnan Purple Rice during Anther Development

During the development of the anthers,potassium antimonite was used to locate Ca~(2+) in fertile and sterile anthers of purple cytoplasmic male sterile rice (Oryza sativa L.ssp.indica).In the fertile anthers,there was abundant Ca~(2+) precipitates on the surface of early microspore,but not in cytoplasm;In the tapetum,there were little calcium precipitates in cytoplasm in the early stage,in the later stage,tapetum cells suffered programmed cell death and a great quantity of calcium was precipitated.In the sterile anthers,the pollen mother cell aborted at the meiosis stage and abundant calcium was precipitated in vacuolated cytoplasm.There were abundant Ca~(2+) preci-(pitates) between tapetum and pollen mother cells or between tapetum and microspores,but not in the tapetum cells.The results indicate that abnormalities in the distribution of calcium between tapetum and pollen mother cells,which is the same between tapetum and microspore,are correlate with cytoplasmic male sterility.