丹参离体微繁技术研究

以丹参(Salvia miltiorrhiza Bunge)离体幼茎、叶、叶柄为外植体，对其丛生芽、不定芽的诱导和增殖、生根、移栽等方面进行系统研究，探讨了有关丹参的离体快速微繁技术。试验表明：MS 6-BA1．0mg／L是诱导初代培养的芽产生丛生芽的最佳培养基，其诱导生芽率为100％，丛生芽增殖的最佳培养基为MS 6-BA1．0mg／L NAA0．01mg／L；以叶为外植体，用MS 6-BA 0．5～2．0mg／L诱导不定芽可取得较好效果，其诱导生芽率为100％，不定芽增殖的最佳培养基为MS 6-BA1．0mg／L，其增殖倍数达24倍；诱导生根较好的培养基为1／2MS 0．1mg／L IBA，移栽先水培再土培，成活率可达100％。

Research on Propagative Technique of Salvia miltiorrhiza Bunge in vitro

Using the young stem, leaf, and leaf-stalk of TBSalvia miltiorrhiza Bunge as the explants, the induction and multiplication of bush-bud and adventitious bud, rooting induction and transplanting are systematically studied, a series of practical propagation techniques of Salvia miltiorrhiza Bunge in vitro are developed. The results showed: MS+6-BA 1.0 mg/L is most suitable for induction of bush-bud with the 100% rate of induction , its best multiplication medium is MS+6-BA 1.0 mg/L+NAA 0.01 mg/L with 15 times of breeding;MS+6-BA 0.5-(2.0 mg/L) is better for inducing adventitious-bud, on which its rate of induction is 100%, its best multiplication medium is MS+6-BA 1.0 mg/L with 24 times of breeding;the better medium for rooting induction is 1/2MS+0.1 mg/L IBA,seedlings should be cultured several days by water before by soil, the 100% living rate of seedling can be obtained.