培养基成分对杜仲愈伤组织生长及次生代谢产物含量的影响

以Bs＋0．5mg／L NAA＋0．5mg／L BA为基本培养基，研究了B5培养基中8种主要无机盐浓度对杜仲愈伤组织生长及绿原酸和总黄酮两种次生代谢产物含量的影响。结果表明：在1000～5000mg／L范围内增加培养基中KNO3的含量有利于愈伤组织生长，B5培养基中当KNO3的浓度达到2／3时，绿原酸和总黄酮含量及产量最高；(NH4)2SO4以4／3原浓度时对愈伤组织生长量、总黄酮含量及产量最高，对绿原酸的含量则是其为原浓度的1／3时最高；MgSO4以2／3浓度对生长量及1／3浓度对绿原酸、总黄酮积累最高；NaH2PO4、CaCl2和MnSO4以原浓度的愈伤组织生长和次生代谢产物合成最好；ZnSO4和FeSO4的原浓度愈伤组织的生长量最大，而1／3浓度的绿原酸和总黄酮含量最高。

Effects of Medium Concentration on Callus Growth and Secondary Metabolites of Eucommia ulmoides

The effects of medium concentration on callus growth and the accumulation of chlorogenic acid and flavonoids of Eucommia ulmoides were investigated. The treatments were designed in B_5 medium supplemented with 0.5 mg/L NAA + 0.5 mg/L BA.The results showed that the callus growth was increased when KNO_3 content was increased,whereas chlorogenic acid and flavonoids contents were highest when KNO_3 concentration was 2/3 of original amount in B_5 medium.The 4/3 content of (NH_4)_2SO_4 in B_5 medium were best for the callus growth and flavonoids content and yield, whereas chlorogenic acid content was highest when (NH_4)_2SO_4 content was 1/3 of original amount in B_5 medium.The callus growth was highest when MgSO_4 concentration was 2/3,whereas chlorogenic acid and flavonoids contents were highest when MgSO_4 content was 1/3 in B_5 medium. The original content of NaH_2PO_4,CaCl_2 and MnSO_4 in B_5 medium were best for the callus growth and the accumulation of chlorogenic acid and flavonoids. The contents of ZnSO_4 and FeSO_4 in B_5 medium was best for the callus growth but chlorogenic acid and flavonoids contents was highest when ZnSO_4 and FeSO_4 concentration was 1/3 in B_5 medium.