红莲型和野败型水稻细胞质雄性不育系线粒体DNA(mtDNA)的比较研究

以水稻红莲型和野败型细胞质雄性不育系为材料,用不连续蔗糖梯度离心法提纯线粒体,以饱和酚-氯仿-异戊醇法抽提mtDNA。用琼脂糖电泳和电镜观察比较不育系mtDNA差异,发现不育系中有小分子mtDNA(即m_2和m_3),不同细胞质间的小分子mtDNA存在差异。在不育系与保持系间名为m_1的大分子mtDNA无差异;本文还研究了mtDNAm_1的理化性质。

COMPARATIVE STUDY ON MITOCHONDRIAL DNA FROM CYTOPLASMIC MALE STERILE LINES OF HONGLIAN TYPE AND YIEBAI TYPE RICE

In the experiments the cytoplasmic male sterility lines from Honglian type and Yiebai type rice were used. Chloroplasts were purified by discontinous sugar density gradiet centrifugation, and mtDNAs were isolated with saturated phenol-chloroferm-iso-Amyl alcohol method. In comparative study on those mtDNAs, it is indicated that there were some small cycle mtDNAs named m_2, m_3 and these small cyele mtDNAs were different between two cytoplasm types based on agarose gel electrophores and electron microscope observation. No difference was founded in the great-molecular mtDNAs named m_3 between sterile and maintainer lines. The experiments also dealt with biochemistic character of mtDNA m_A.