利用激光扫描共聚焦显微镜研究拟南芥气孔发生与发育

通过激光扫描共聚焦显微镜,利用不同种类(波长)的激光研究拟南芥叶片气孔发生与发育。结果表明,利用紫外激光(351nm)扫描可以清楚观察到拟南芥表皮各种细胞及其发生发育的形态变化,包括表皮毛细胞、副卫细胞、保卫细胞、铺垫表皮细胞等。气孔发生过程中,首先原表皮细胞不对称分裂产生拟分生组织和副卫细胞,接着分化出保卫细胞母细胞,进一步发育形成保卫细胞,最终形成气孔器。气孔分化完成后,保卫细胞在紫外激光下不产生荧光,但利用蓝光激发(488nm)辅助荧光素染色,可清晰地看到保卫细胞。结果表明,激光扫描共聚焦显微镜在拟南芥叶表皮细胞形态研究上有独特的功能。

Studies on Morphogenesis and Development of Arabidopsis thaliana Stomata Using Confocal Laser Scanning Microscope

The morphogenesis and development of lamina stomata in Arabidopsis thaliana was studied,usingconfocal laser scanning microscope/Uhe results showed that all types of cells,such as trichomes,subsidiary,guard and epidermal pavement cells,and their morphogenesis variation were observed clearly under UV laser(351 nm).During the formation of stomata,one protodermal cell produced a meristemoid and subsidiary cells by asymmetric division,followed by the formation of guard mother cell,which became two guard cells.Stomata were terminally differentiated.After the formation of stomata,guard cells could not emit fluorescence under UV laser(351 nm).So blue laser(488 nm)with staining by fluorescein was adopted and guard cells were seen clearly.It indicated that CLSM was a very novel tool to study the morphogenesis and development of lamina stomata.