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| 强德勒红心柚离体培养与植株再生体系的建立 | |
| 引用本文： | 曾艳,苏智先,邹利娟,吴庆贵,毛艳萍,程昌凤.强德勒红心柚离体培养与植株再生体系的建立[J].武汉植物学研究,2010,28(2):218-223. |
| 作者姓名： | 曾艳 苏智先 邹利娟 吴庆贵 毛艳萍 程昌凤 |
| 作者单位： | 1. 西华师范大学生命科学学院,四川南充,637002四川省生态与环境技术重点实验室,四川绵阳,621000 2. 四川省生态与环境技术重点实验室,四川绵阳,621000 3. 重庆市农业科学院果树研究所,重庆江津,402260 |
| 基金项目： | 四川省教育厅项目，四川省绵阳市科技攻关项目 |
| 摘 要： | 以强德勒红心柚（Citrus grandis Osbeckcv. Chandler）种子萌发的无菌苗为材料,选取子叶、子叶节段、上胚轴、带芽的茎段进行离体培养研究。结果表明：子叶节段是诱导丛生芽的最佳外植体,诱导率100%;诱导丛生芽的最佳培养基为MS＋6-BA2.0mg/L＋NAA0.05mg/L＋蔗糖30g/L＋活性炭0.4g/L,丛生芽增殖可达11.2倍;最适生根培养基为1/2MS＋NAA0.5mg/L,生根率达100%,移栽15d后成活率100%。 |
| 关 键 词： | 强德勒红心柚 离体培养 丛生芽增殖 |
Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv. Chandler |
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| ZENG Yan,SU Zhi-Xian,ZOU Li-Juan,WU Qing-Gui,MAO Yan-Ping,CHENG Chang-Feng.Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv. Chandler[J].Journal of Wuhan Botanical Research,2010,28(2):218-223. | |
| Authors: | ZENG Yan SU Zhi-Xian ZOU Li-Juan WU Qing-Gui MAO Yan-Ping CHENG Chang-Feng |
| Institution: | ZENG Yan1,2,SU Zhi-Xian2,ZOU Li-Juan2,WU Qing-Gui2,MAO Yan-Ping1,CHENG Chang-Feng3(1.College of Life Science,China West Normal University,Nanchong,Sichuan 637002,China,2.Sichuan Provincial Key Laboratory of Ecology , Environmental Technology,Mianyang,Sichuan 621000,3.Fruit Research Institute,Chongqing Academy of Agricultural Science,Jiangjin,Chongqing 402260,China) |
| Abstract: | The cotyledons,cotyledonary nodes,epicotyl segments,and stem segments with lateral buds of aseptic Citrus grandis Osbeck cv.Chandler seedings were used as the explants to study the process of tissue culture rapid reproduction.Results showed that the cotyledonary nodes were the best explants of bush-bud induction(induction rate 100%).The best medium for inducing bush-bud was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+sucrose 30 g/L+activated carbon 0.4 g/L,the coefficient of proliferation reached 11.2.The optimum medium... |
| Keywords: | Citrus grandis Osbeck cv Chandler Culture in vitro Bush-bud regeneration |
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