药用植物金荞麦愈伤组织诱导与植株再生

目前转基因技术已成为植物定向遗传改良的重要手段,而建立稳定高频的离体再生系统是实现遗传转化的基础和前提.本试验以25 ～30 d苗龄的金养麦(Fagopyrum dibotrys)无菌苗叶片、茎节间、叶柄为外植体进行愈伤组织诱导与植株再生研究.结果表明:叶片在MS +2,4-D 4.0 mg/L +6-BA 1.0 mg/L培养基上愈伤组织诱导率达到89％.茎节间在MS +2,4-D 2.0 mg/L +6-BA 2.0 mg/L培养基上愈伤组织诱导率为87％.叶柄在MS +2,4-D 4.0 mg/L +6-BA 2.0 mg/L+ IBA 0.2 mg/L培养基上的最高诱导率仅为54％.愈伤组织分化不定芽的适宜培养基为MS +6- BA2.0 mg/L +TDZ0.2 mg/L +NAA0.2 mg/L;金荞麦不定芽在1/2 MS +NAA 0.5 mg/L的培养基上生根效果最好.组培再生植株经炼苗后移栽到田间成活率达80％以上,且生长表现正常.高频完整再生体系的建立,为金荞麦进一步遗传操作和扩大药材资源奠定了基础.

Callus Induction and Plant Regeneration of Fagopyrum dibotrys (D.Don) Hara, An Important Medicinal Plant

Transgenic technology has now become one of the most important measures in plant directional genetic improvement,while the establishment of a high-efficiency regenerating system must be the basis and prerequisite of genetic transformation.In the present research,explants（leaf,petiole and stem segments） excised from 25-30 day-old in vitro seedlings of Fagopyrum dibotrys were used as materials for inducing callus and plant regeneration.Results showed that MS＋2,4-D 4.0 mg/L＋6-BA 1.0 mg/L was beneficial for leaves to form callus（89%）.Internodal cuttings formed callus（87%） on MS medium supplemented with 2,4-D 2.0 mg/L＋6-BA 1.0-2.0 mg/L,and the highest callus induction from the petiole was only 54% on MS＋2,4-D 4.0 mg/L＋6-BA 2.0 mg/L＋IBA 0.2 mg/L media.The medium of MS＋6-BA 2.0 mg/L＋TDZ 0.2 mg/L＋NAA 0.2 mg/L was prior to callus differentiation and shoot organogenesis.The optimum medium for producing roots（96.67%） was half-strength MS medium supplemented with NAA（0.5 mg/L）.Hardened plantlets via acclimatization were successfully transplanted to a field（80%）,with the regenerated plants normal morphologically and in growth characters.The high-efficient stable organogenesis regeneration system of Fagopyrum dibotrys will make it possible for genetic transformation and enlarging medical resources for this species.