| 用显微分光光度术测定不同发育时期蚕豆子叶淀粉质体DNA含量 |
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| 引用本文： | 孙德兰,白雪芳.用显微分光光度术测定不同发育时期蚕豆子叶淀粉质体DNA含量[J].武汉植物学研究,1996,14(1):71-76. |
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| 作者姓名： | 孙德兰 白雪芳 |
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| 作者单位： | 中国科学院植物研究所，中国科学院大连化学物理研究所 |
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| 摘 要： | 叙述了用显微光度术在亚细胞水平上测量蚕豆子叶细胞中淀粉质体Ｆｅｕｌｇｅｎ－ＤＮＡ含量，显示了在不同发育时期蚕豆子叶细胞中的淀粉体ＤＮＡ含量的动态变化。随着发育时期的递增，淀粉体ＤＮＡ拷贝数不断地增长。生长中期的淀粉体ＤＮＡ含量平均数为２．１４（任意单位），到了成熟期淀粉体ＤＮＡ平均含量增长到１０．１２（任意单位）。在成熟期，子叶细胞中的淀粉体ＤＮＡ含量比生长中期增长了９．６倍。通过生物统计分析，在
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| 关 键 词： | 蚕豆子叶 淀粉体DNA |
| 收稿时间： | 1994-10-11 |
| 修稿时间： | 1995-3-4 |
MICROPHOTOMETRIC DETERMINED OF AMYLOPLASTS DNA CONTENT OF DIFFERENT DEVELOPMENTAL PERIODS IN COTYLEDON CELL OF BROAD BEAN |
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| Sun Delan,Bai Xuefang.MICROPHOTOMETRIC DETERMINED OF AMYLOPLASTS DNA CONTENT OF DIFFERENT DEVELOPMENTAL PERIODS IN COTYLEDON CELL OF BROAD BEAN[J].Journal of Wuhan Botanical Research,1996,14(1):71-76. |
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| Authors: | Sun Delan Bai Xuefang |
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| Institution: | 1. Institute of Botany, The Chinese Academy of Sciences Beijing 100044;2. Dalian Institute of Chemical Physics, The Chinese Academy of Sciences Dalian 116023 |
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| Abstract: | The Feulgen-DNA contents of amyloplasts in cotyledon cell of broad bean in sub-cellular level have been determined by microphometry and the result showed the dynamic change in different developmental periods. The amyloplasts DNA of copies increase with the developmental stage-The average of amyloplasts DNA content is 2.14(arbitrary unit) in the middle stage of growth. Amyloplasts DNA increase to 10.12 (arbitrary unit) in the mature period of cotyledon cell of broad bean. Amyloplasts DNA content in cotyledon cell of mature period is 9.6 times than that in the middle period of growth.The change of variability DNA contents among plastids showed normal distribution in natural logarithm by biological statistics. |
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| Keywords: | Broad bean cotyledon Amyloplast DNA |
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