红菜薹雄性不育系花药败育的细胞形态学观察

采用石蜡切片技术，在光学显微镜下系统研究了红菜薹(Brassica campestris L.ssp．chinensis L.var．utilis TsenetLee．)波里马胞质雄性不育系(Polima CMS)、红菜薹萝卜胞质雄性不育系(Ogura CMS)及相应保持系花药发育过程的细胞形态学特征。观察结果表明：红菜薹Polima CMS花药发育受阻于孢原细胞阶段，不形成花粉，属无花粉型，此不育系花药不形成绒毡层和中层；而红菜薹Ogura CMS花药败育发生于小孢子母细胞期或四分体时期，表现为绒毡层细胞异常，挤压四分体，导致四分体和绒毡层同时解体而败育。

The Cytomorphology Study of Anther Abortion in Male-Sterile Lines of Hong Cai-tai (Brassica campestris L.ssp.chinensis L.var.utilis Tsen et Lee.)

Microsporogenesis of Hong Cai-tai's Polima cytoplasmic male-sterile line (Polima CMS),Hong Cai-tai's Ogura cytoplasmic male-sterile line(Ogura CMS) and their maintainers were investigated cytologically by using paraffin section with light microscopy systemly.The observation showed that the anther development of Hong Cai-tai's Polima male-sterile line was blocked without anther at the stage of archesporial cell.The few pollen and no anther cell was observed without forming the tapetum and the middle layer;while the anther development of Hong Cai-tai's Ogura male-sterile line was blocked without anther at the stage of microspore mother cell & tetrads of microspores,observing the tapetum cell abnormal and tetrads of microspores being extruded.In the end the tapetum cell and tetrads of microspores disaggregated.