| 外源DNA导入小麦引起遗传变异的验证 |
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| 引用本文： | 徐乃瑜,严家骐,武军驻.外源DNA导入小麦引起遗传变异的验证[J].武汉植物学研究,2000,18(5):351-355. |
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| 作者姓名： | 徐乃瑜 严家骐 武军驻 |
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| 作者单位： | 武汉大学生命科学学院,武汉,430072 |
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| 基金项目： | 国家“八 .五”科技攻关项目!(编号 :85-0 0 2 -0 2 -0 3)，湖北省“九 .五”农业重大科技项目!(编号 :982 P0 4 0 4 )资助课题 |
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| 摘 要： | 对由波兰小麦ＤＮＡ注射到普通小麦鄂恩１号子房获得的Ｄ５代稳定遗传变异２个转化株系的种子醇溶蛋白，进行单向和双向聚丙烯酰胺凝胶电泳分析，结果表明由外源ＤＮＡ导入获得转化株系的变异，与其种子醇溶蛋白电泳图谱出现供体的某些组分和缺少受体的某些组分相印证。
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| 关 键 词： | 醇溶蛋白 单向和双向电泳 外源DNA导入 转化株系 |
| 收稿时间： | 1999-10-12 |
| 修稿时间： | 2000-4-2 |
VERIFICATION OF THE GENETIC VARIATION INDUCED BY INTRODUCTION OF EXOGENOUS DNA INTO WHEAT BY USING ELECTROPHORETIC PATTERNS ANALYSIS OF SEED GLIADINS |
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| Xu Naiyu,Yan Jiaqi,Wu Junzhu.VERIFICATION OF THE GENETIC VARIATION INDUCED BY INTRODUCTION OF EXOGENOUS DNA INTO WHEAT BY USING ELECTROPHORETIC PATTERNS ANALYSIS OF SEED GLIADINS[J].Journal of Wuhan Botanical Research,2000,18(5):351-355. |
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| Authors: | Xu Naiyu Yan Jiaqi Wu Junzhu |
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| Institution: | College of Life Sciences, Wuhan University Wuhan 430072 |
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| Abstract: | Seed gliadins of the fifth generation (D 5) stably herditary variant types were obtained by injection of the total DNA of Triticum polonicum L.into the ovary of common wheat Triticum aestivum E en 1 and were analyzed by comparison of their one and two dimensional polyacrylamide gel electrophoretic patterns. The results showed that variation of transformation line was obtained by the introduction of exogenous DNA, accompanied with the appearance of some components of donor and the absence of some components of receptor on the electrophoretic patterns of seed gliadins proved each other. |
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| Keywords: | Gliadin One and two dimensional electrophoresis Introduction of exogenous DNA Transformation line |
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