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青花菜细胞质雄性不育系线粒体DNA的提取与RAPD分析
引用本文:朱玉英,杨晓锋,侯瑞贤,李式军,吴琴生.青花菜细胞质雄性不育系线粒体DNA的提取与RAPD分析[J].武汉植物学研究,2006,24(6):505-508.
作者姓名:朱玉英  杨晓锋  侯瑞贤  李式军  吴琴生
作者单位:1. 上海市农业科学院园艺研究所,上海,201106;上海市设施园艺技术重点实验室,上海,201106
2. 南京农业大学园艺学院,南京,210095
基金项目:上海市科技兴农科技攻关项目
摘    要:利用分子生物学方法提取青花菜不育系及其保持系线粒体的DNA,经琼脂糖凝胶电泳表明所提取的线粒体DNA纯度较高。通过40个引物进行随机引物多态性扩增,结果表明,由引物S66扩增出一条1 000 bp左右的特异条带BMS1000,该条带是不育系m tDNA所特有的,初步推断该特异条带可能与不育性相关。

关 键 词:青花菜  雄性不育系  线粒体DNA  RAPD
文章编号:1000-470X(2006)06-0505-04
收稿时间:2006-06-26
修稿时间:2006-06-262006-08-22

Extraction and RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile (CMS) Line in Broccoli
ZHU Yu-Ying,YANG Xiao-Feng,HOU Rui-Xian,LI Shi-Jun,WU Qin-Sheng.Extraction and RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile (CMS) Line in Broccoli[J].Journal of Wuhan Botanical Research,2006,24(6):505-508.
Authors:ZHU Yu-Ying  YANG Xiao-Feng  HOU Rui-Xian  LI Shi-Jun  WU Qin-Sheng
Institution:1. Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2. Shanghai Key Lab. of Protected Horticultural Technology, Shanghai 201106, China; 3. Department of Horticulture, NanjingAgri. University, Nanjing 210095, China
Abstract:DNA from the mitochondrion(mtDNA) of CMS line BC7-19 and its maintaner of broccoli(Brassica oleracea L.var.italica Plenck.) was extracted by molecular bio-technique,and was marked(using) RAPD reaction.The result showed that the purity of the extracted mtDNA was high by sepharose(electrophoresis).And the result also showed that a special fragment,BMS_(1000),with about(1000) bp amplificated by primer S66,was peculiar for the mtDNA of male sterile line by RAPD reaction of 40 primers,and the special fragment was initially considered to be relevant to male sterility after sequence analysis.
Keywords:Broccoli(Brassica oleracea L  var  italica Plenck  )  Male sterile line  mtDNA  RAPD
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