| 洋紫荆凝集素的分离纯化和性质 |
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| 引用本文： | 彭建宗,陈兆平,程双奇.洋紫荆凝集素的分离纯化和性质[J].植物学报,2000,17(3):266-269. |
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| 作者姓名： | 彭建宗 陈兆平 程双奇 |
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| 作者单位： | (Department of Biology, South China Normal University, Guangzhou 510631) |
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| 摘 要： | 利用酸化处理的Sepharose 6B 亲和柱从洋紫荆种子中分离纯化出了洋紫荆凝集素(BVL)，其比活性比抽提液提高了159倍，活力回收率为49.0%。BVL分子量为81 000，由两个相同的亚基组成。等电聚焦凝胶电泳测得其等电点为4.95。其紫外吸收高峰在276 nm处。BVL具一定的热稳定性和酸碱稳定性。N-乙酰-D-氨基半乳糖能强烈地抑制BVL对兔红细胞的凝集作用，乳糖、半乳糖也有较强的抑制作用。
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| 关 键 词： | 凝集素 洋紫荆 亲和层析 纯化 |
| 收稿时间： | 1999-04-01 |
| 修稿时间： | 1999-12-09 |
Purification and Characterization of Lectin from Bauhinia variegata L. |
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| PENG Jian-Zong,CHEN Zhao-Ping,CHENG Shuang-Qi.Purification and Characterization of Lectin from Bauhinia variegata L.[J].Bulletin of Botany,2000,17(3):266-269. |
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| Authors: | PENG Jian-Zong CHEN Zhao-Ping CHENG Shuang-Qi |
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| Abstract: | Bauhinia variegata lectin（BVL） has been purified from the seed of Bauhinia variegata L. by affinity chromatography on an acid treated Sepharose 6B column . The hemagglutinating activity of the purified BVL increased 159 times and the activity recovery is 49.0%. The molecular weight is 81 000 and the molecule consists of two identical subunits. Its isoelectric point is 4.95. Nacetyl Dgalactosamine is the most potent inhibitors of BVL. Lactose and galactose can also inhibit the agglutination of rabbit erythocytes by BVL. |
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| Keywords: | , |
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