| 山西糜子核心种质分子身份证构建 |
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| 引用本文： | 王宇卓,林元香,薛亚鹏,段政勇,王晓丹,陈凌,曹晓宁,王瑞云,乔治军.山西糜子核心种质分子身份证构建[J].植物学报,2023,58(1):159-168. |
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| 作者姓名： | 王宇卓 林元香 薛亚鹏 段政勇 王晓丹 陈凌 曹晓宁 王瑞云 乔治军 |
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| 作者单位： | 1山西农业大学农学院, 太谷 0308012山西农业大学农业基因资源研究中心, 农业农村部黄土高原作物基因资源与种质创制重点实验室, 杂粮种质资源发掘与遗传改良山西省重点实验室, 太原 030031 |
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| 基金项目： | 国家自然科学基金(31271791);现代农业产业技术体系建设专项资金(CARS-06-14.5-A16);山西省研究生创新项目(2022Y331);山西省重点研发项目(2022ZDYF110) |
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| 摘 要： | 为快速鉴定糜子(Panicum miliaceum)资源, 建立分子标记检测平台, 以272份山西糜子核心种质为研究材料, 利用85对简单重复序列(SSR)引物, 应用ID Analysis 4.0软件, 构建DNA分子身份证。结果表明, 对85对SSR引物进行筛选, 发现20对引物组合(RYW67、RYW53、RYW37、RYW65、RYW62、RYW77、RYW5、RYW49、RYW84、RYW19、RYW11、RYW40、RYW54、RYW28、RYW31、RYW7、RYW16、RYW8、RYW9和RYW18)可区分272份材料。共检测到等位变异(Na) 60个, 平均每个位点检出3个; Shannon多样性指数(I)为0.957 8 (RYW16)-1.096 7 (RYW5), 平均值为1.055 2; 多态性信息含量(PIC)为0.604 4 (RYW77)-0.753 0 (RYW37), 平均值为0.692 1。利用20对引物构建山西糜子核心种质的字符串、条形码和二维码DNA分子身份证, 可为种质身份标识和溯源提供实践路径。
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| 关 键 词： | 糜子 核心种质 分子身份证 山西 简单重复序列 |
| 收稿时间： | 2022-08-06 |
Construction of Molecular ID Card of Core Germplasm of Hog Millet (Panicum miliaceum) in Shanxi |
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| Yuzhuo Wang,Yuanxiang Lin,Yapeng Xue,Zhengyong Duan,Xiaodan Wang,Ling Chen,Xiaoning Cao,Ruiyun Wang,Zhijun Qiao.Construction of Molecular ID Card of Core Germplasm of Hog Millet (Panicum miliaceum) in Shanxi[J].Bulletin of Botany,2023,58(1):159-168. |
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| Authors: | Yuzhuo Wang Yuanxiang Lin Yapeng Xue Zhengyong Duan Xiaodan Wang Ling Chen Xiaoning Cao Ruiyun Wang Zhijun Qiao |
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| Institution: | 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, China2Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs, Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China |
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| Abstract: | In order to quickly identify Panicum miliaceum resources, a molecular label/ID detection platform was established. In this experiment, 272 core germplasms of hog millet in Shanxi province were used as research materials, and 85 pairs of SSR primers were used as detection tools. ID Analysis 4.0 software was used to construct the DNA molecular ID of the materials. The main results were as follows: out of the 85 pairs of SSR primers that were screened, and 20 pairs of primer combinations (RYW67, RYW53, RYW37, RYW65, RYW62, RYW77, RYW5, RYW49, RYW84, RYW19, RYW11, RYW40, RYW54, RYW28, RYW31, RYW7, RYW16, RYW8, RYW9, and RYW18) could distinguish 272 materials. A total of 60 alleles (Na) were detected, with an average of 3 distinct alleles per locus. Shannon diversity index (I) was 0.957 8 (RYW16)-1.096 7 (RYW5), with an average of 1.055 2. The polymorphism information content (PIC) was 0.604 4 (RYW77)-0.753 0 (RYW37), with an average of 0.692 1. The 20 pairs of primers were used to construct the string, bar code and two-dimensional code DNA molecular identity card of Shanxi hog millet core germplasm, providing a practical way for germplasm identification and traceability management. |
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| Keywords: | hog millet core germplasm molecular identity card Shanxi simple sequence repeat |
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