花生微卫星标记的研究进展

近年来花生微卫星标记的开发取得了一定的进展, 初步揭示了花生在DNA水平上的遗传多样性。花生微卫星标记的开发途径主要包括通过构建小片段基因组文库开发基因组SSR标记, 根据花生EST序列开发EST-SSR标记, 根据豆科植物序 列信息和SSR标记开发花生SSR标记, 将SSR标记与其它分子标记结合开发新的DNA标记, 以及基于SSR核心序列开发ISSR标记。花生微卫星标记主要应用于遗传多样性研究、遗传图谱与品种指纹图谱构建以及分子标记辅助育种等领域。本文综述了花生SSR标记开发研究的进展及应用。

Advances in Study of Microsatellite Markers in Groundnut

In recent years, with the discovery of microsatellite markers in groundnut (Arachis hypogaea L.), the genetic diversity of this crop has been extensively investigated by analysis of simple sequence repeats (SSR). The approaches to developing groundnut microsatellite markers included development of SSR markers from genomic libraries constructed with small DNA fragments, development of expressed sequence tag (EST)-SSR markers from EST sequences generated from different cDNA libraries, identification of transferable SSR markers from other legume crops, development of new markers by combining other markers with SSR markers and generation of inter-SSR (ISSR) markers based on SSR motifs. Groundnut microsatellite markers have been applied in genetic diversity analysis, linkage map construction, fingerprinting analysis of cultivars and marker-assisted breeding. This review summarizes the progress in development of peanut SSR and the application of these markers.