| 基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用 |
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| 引用本文： | 何晓玲,刘鹏程,马伯军,陈析丰.基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用[J].植物学报,2022,57(4):508-531. |
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| 作者姓名： | 何晓玲 刘鹏程 马伯军 陈析丰 |
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| 作者单位： | 浙江师范大学化学与生命科学学院, 金华 321004 |
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| 基金项目： | 国家自然科学基金(31871605);国家自然科学基金(32071987);国家自然科学基金(32101697);浙江省自然科学基金(LD19C130001);浙江省自然科学基金(Q22C133851) |
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| 摘 要： | CRISPR/Cas9技术是利用RNA靶向引导Cas9核酸酶对基因组中的目标基因进行编辑的生物技术。近年来, 该技术的多种新型基因编辑器更新迅猛, 编辑效果愈加精细和高效, 在作物定向分子设计育种中展现出巨大的应用前景。该文对CRISPR/Cas9及其相关编辑器的技术原理、编辑效果和应用情况进行综述, 并探讨了该技术在应用中面临的问题、应对措施和发展前景, 旨在为相关领域的科研工作者提供参考。
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| 关 键 词： | CRISPR/Cas9 基因编辑 技术进展 在植物中应用 |
| 收稿时间： | 2022-01-21 |
Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants |
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| He Xiaoling,Liu Pengcheng,Ma Bojun,Chen Xifeng.Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants[J].Bulletin of Botany,2022,57(4):508-531. |
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| Authors: | He Xiaoling Liu Pengcheng Ma Bojun Chen Xifeng |
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| Institution: | College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China |
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| Abstract: | CRISPR/Cas9 technology is a gene editing strategy using Cas9 nuclease guided by RNA to target an interest gene in genome. Recently, a large number of new-type gene editors based on the CRISPR/Cas9 have been updated rapidly and become more precisely and efficiently in gene editing, which has a great application prospect in crop molecular-designing breeding. This paper summarized the technical principles, editing effects and applications of the CRISPR/cas9 and its related gene editors, and also discussed the aspects of dilemmas, countermeasures and prospects, intending to provide reference for scientific researchers in the related fields. |
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| Keywords: | CRISPR/Cas9 gene editing technical progress application in plants |
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