广东高州7个普通野生稻居群遗传结构的SSR分析

利用32对SSR引物对来自全部7个自然居群的217份广东高州普通野生稻(简称“高野”)材料进行遗传结构、多样性和遗传聚类分析。结果表明, 高野各居群因遗传结构存在差异而相对独立, 但各居群之间由于存在基因渗透又具有一定的相似性。高野总体多样性指数(Ht)为0.65, 居群内的多样性(HS=0.431)略大于居群间的多样性(DS=0.392), 二者差异并不显著。居群间的遗传分化系数(GST)为0.611, 说明高野群体的遗传差异是由居群内和居群间的遗传分化共同作用的结果。其中A、B、E居群间, D、F、G居群间遗传相似性较高, C居群与其它居群之间存在较大差异。根据7个居群的遗传结构, 结合其地理分布状况, 认为遗传多样性最大的B和E居群以及遗传分化最小的C居群应作为重点对象进行保护。

Analysis of Genetic Structure by Simple Sequence Repeat Markers in Seven Oryza rufipogon Griff. Populations from Gaozhou

The genetic structure of 217 individuals from seven natural populations of oryza rufipogon Griff. in Gaozhou was analyzed by use of 32 pairs of simple sequence repeat(SSR) primers. The populations differed in genetic structure but were also similar to some extent because of genetic introgression. The genetic diversity index of all populations(Ht) was 0.65; the diversity within populations(HS=0.431) was slightly higher than that among populations(DS=0.392), and the coefficient of genetic differentiation(GST) was 0.611. Thus, the genetic differentiation of o. rufipogon Griff. in Gaozhou was caused by differentiation within and among populations. The level of genetic similarity was high among populations A, B, and E and among populations D, F, and G; the genetic difference between population C and other populations was large. Considering the geographic distributions of the populations with the above results, population C, with the minimum genetic differentiation, and populations B and E, with the maximum genetic diversity, should be conserved before others.