首页 | 本学科首页   官方微博 | 高级检索  
   检索      

转基因红花中角质细胞生长因子KGF-1的表达
引用本文:江莺,刘秀明,马吉胜,李巍,朱海林,杜美丽,李海燕,李校堃.转基因红花中角质细胞生长因子KGF-1的表达[J].植物学报,2011,46(3):311-318.
作者姓名:江莺  刘秀明  马吉胜  李巍  朱海林  杜美丽  李海燕  李校堃
作者单位:吉林农业大学生物反应器与药物开发教育部工程研究中心, 长春 130118;
吉林农业大学中药材学院, 长春 130118
吉林农业大学生命科学学院, 长春 130118
基金项目:863计划,吉林省科技发展计划重点项目,高等学校科技创新工程重大项目培育资金项目
摘    要:通过构建重组表达质粒载体p139035S-KGF1和根癌农杆菌介导在红花(Carthamus tinctorius)中表达角质细胞生长因子(KGF-1)。从侵染到诱导生根共需要14周, 转化率达0.1%。红花子叶在潮霉素筛选培养基上培养4–5周后便可获得丛生芽, 再生芽移入含潮霉素的伸长生根培养基, 培养4–8周可诱导生根。通过PCR、Southern blot、RT-PCR及Western blot检测证明目的基因KGF-1已经整合到红花细胞的染色体中, 实现了KGF-1外源蛋白在红花中的成功表达, 为开发KGF-1蛋白新的生产途径奠定了基础。

收稿时间:2010-12-01
修稿时间:2011-01-24

Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower
Ying Jiang,Xiuming Liu,Jisheng Ma,Wei Li,Hailin Zhu,Meili Du.Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower[J].Bulletin of Botany,2011,46(3):311-318.
Authors:Ying Jiang  Xiuming Liu  Jisheng Ma  Wei Li  Hailin Zhu  Meili Du
Institution:Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University, Changchun 130118, China;

College of Chinese Medicinal, Jilin Agricultural University,Changchun 130118, China;

College of Life Sciences, Jilin Agricultural University, Changchun 130118, China
Abstract:The recombinant plasmid vector p139035S-KGF1 was constructed and transfected into safflower by Agrobacterium tumefaciens transfection. The period of infection to rooting was 14 weeks, and transformation efficiency was about 0.1%. Adventitious shoots were generated after cotyledons were cultured in selecting media containing hygromycin for 4–5 weeks. Rooted safflower was obtained in 4–8 weeks. KGF-1 was transformed into safflower chromosomes and expressed successfully, as determined by PCR, Southern blot, RT-PCR and Western blot analysis, which established the basis for the production of KGF-1.
Keywords:
点击此处可从《植物学报》浏览原始摘要信息
点击此处可从《植物学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号