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利用离体叶片鉴定杨树耐盐潜力
引用本文:张颖,杨迎霞,郏艳红,周祥明,聂莉莉,张越,陈受宜,王景安,刘仲齐.利用离体叶片鉴定杨树耐盐潜力[J].植物学报,2011,46(3):302-310.
作者姓名:张颖  杨迎霞  郏艳红  周祥明  聂莉莉  张越  陈受宜  王景安  刘仲齐
作者单位:天津师范大学生命科学学院, 天津 300384;
天津市农业生物技术研究中心, 天津 300192
中国科学院遗传与发育生物学研究所, 北京 100101
基金项目:973计划,"十一五"国家科技支撑计划
摘    要:以受体杨树107和转基因杨树18-1的一年生枝条为材料, 采用Hoagland营养液水培方法, 添加不同浓度的NaCl, 检测二者植株生长以及叶中Na+和K+含量的变化。结果表明, 在0.6%NaCl处理下18-1生根率明显高于107, 生物量较大, 叶片中Na+积累量是107的1.45倍左右。以107和18-1离体叶片为材料, NaCl处理下测定其生理活性指标, 发现18-1叶盘的失绿速度和相对电导率都显著低于107叶盘。把离体叶片接种在改良MS培养基上, 1.2%NaCl处理可使107叶盘几乎停止伸长, 细胞大小不再增加; 而18-1叶盘培养7天后伸长了近50%。以上结果表明利用离体叶片可以鉴定出不同基因型杨树的耐盐潜力。

收稿时间:2010-10-08
修稿时间:2011-01-21

Using Leaf Sections to Identify Different Salt Tolerance Potential of Poplar Plants
Ying Zhang,Yingxia Yang,Yanhong Jia,Xiangming Zhou,Lili Nie,Yue Zhang,Shouyi Chen,Jingan Wang,Zhongqi Liu.Using Leaf Sections to Identify Different Salt Tolerance Potential of Poplar Plants[J].Bulletin of Botany,2011,46(3):302-310.
Authors:Ying Zhang  Yingxia Yang  Yanhong Jia  Xiangming Zhou  Lili Nie  Yue Zhang  Shouyi Chen  Jingan Wang  Zhongqi Liu
Institution:College of Life Sciences, Tianjin Normal University, Tianjin 300384, China;

Tianjin Research Center of AgriculturalBio-technology, Tianjin 300192, China;

Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China
Abstract:Plant dry weight and changes in Na+ and K+ contents in leaves were investigated in 1-year-old cuttings from the receptor poplar 107 (Populus × euramericana ‘Neva’) and transgenic poplar 18-1 after cuttings were cultured in Hoagland solution with different NaCl concentrations. The growth and rooted percentage of 18-1 was higher than that of 107 with 0.6%NaCl, and the accumulation of Na+ in 18-1 leaves was about 1.45 times higher than that in 107. Physiological characteristics in leaf sections from 107 and 18-1 under NaCl stress were determined. Leaf sections from 18-1 displayed lower chlorophyll decreasing speed and relative electrical conductivity than that from 107. When sections of young poplar leaves were plated on MS medium with 1.2%NaCl, leaf sections from 107 stopped growing, but those from 18-1 grew continuously and their length increased nearly 50% after 7 days. Thus, salt tolerance between different genotypes can be identified in leaf sections.
Keywords:
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