甘薯叶柄原生质体有效植株再生

将甘薯(Ipomoea batatas (L．)Lam．)‘元气’和‘白星’(‘White Star’)的叶柄原生质体培养在含有0.05 mg·L-1 2,4-D和0.5 mg·L-1 KT的改良MS液体培养基中，3～4 d后细胞开始分裂。培养8～9周后，将直径达1～2 mm的愈伤组织转移到添加0.05～0.2 mg· L-1 2,4-D和0～0.5 mg·L-1 KT或添加0.5～2.0 mg ·L-1 NAA和1.0～3.0 mg·L-1 BAP 的MS固体增殖培养基上使其增殖。转移3～5周后，将愈伤组织再转移到MS基本培养基或转移到添加2.0～3.0 mg·L-1 BAP的MS培养基上。当进一步转移到MS基本培养基上后，从愈伤组织或从愈伤组织形成的不定根上再生出植株。‘元气’植株再生率高达60.0 ％，White Star高达43.4％。

Efficient Plant Regeneration from Potiole Protoplasts of Sweet Potato

Protoplasts isolated from petioles of sweet potato ‘Cenki’ and ‘White Star’ were cultured in the modified liquid MS medium containing 0.05 mg· L- 1 2,4-D and 0.5 mg· L- 1 KT. The first cell division occurred within 3 to 4 days. After 8 to 9 weeks of culture, small calli derived from protoplasts were placed on the solid MS medium supplemented either with 0.05-0.2 mg· L-1 2,4-D and 0-0.5 mg·L- 1 KT or with 0.5-2.0 mg·L -I NAA and 1.0-3.0 mg·L- 1 BAP for callus proliferation. Furthermore, the calli were transferred on to either the hormone-free medium or the medium supplemented with 2.0-3.0 mg· L- l BAP, followed by being transferred on to the hormone-free medium for plant regeneration. The frequency of protoplast-derived calli regenerating plants reached 60.0% in ‘Genki’ and 43.3% in 'White Star.