砷超富集植物蜈蚣草原生质体的分离及其抗砷性分析

蜈蚣草(Pteris vittata)是一种砷超富集植物, 能够通过根从土壤中吸收砷, 并将其输送至羽叶中富集。为了探索蜈蚣草单个细胞在砷积累和砷抗性中的特性, 本文首次通过酶解方法获得了这一砷超富集蕨类植物的原生质体, 并研究了原生质体在不同浓度砷胁迫下的生活力。结果显示, 蜈蚣草原生质体的抗砷性远高于烟草原生质体的抗砷性, 与其整体植株的抗性一致。这为探索砷抗性和超富集机理提供了一个新的研究体系。

Isolation and Analysis for Arsenic Tolerance of Protoplasts from an Arsenic Hyperaccumulator Pteris vittata

Pteris vittata was the first identified arsenic (As) hyperaccumulator. Because of this characteristic, it is a good species for studying the acclimatization of plants to environmental stress, especially research on the mechanism of hyperaccumulation and resistance to As. Besides being able to absorb As from soil to root, the species also possesses a highly efficient root-to-shoot transport system for the metalloid, which results in a large ratio of shoot-to-root As concentration. To understanding the resistance of the fern cells to As and comparison to other plants that do not possess the hyperaccumulator characteristics, we studied the conformation of the protoplast and its resistance to As. Protoplasts of the fern were extracted with use of enzymes. The resistance of the fern protoplast surpasses that of tobacco; thus, we have revealed a faster system for identifying resistance to metalloids.