| 利用毛细管电泳法分析甜菊糖苷的含量 |
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| 引用本文： | 邵寒娟,胡涌刚,丁亮,沈明山,陈睦传,张向红.利用毛细管电泳法分析甜菊糖苷的含量[J].植物学报,2001,18(1):113-117. |
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| 作者姓名： | 邵寒娟 胡涌刚 丁亮 沈明山 陈睦传 张向红 |
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| 作者单位： | １（厦门大学生命科学院细胞生物学与肿瘤细胞工程教育部重点实验室 厦门 361005) ２(厦门大学化学系固体表面物理化学国家重点实验室 厦门 361005) |
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| 摘 要： | 本文介绍了一种用毛细管区带电泳法筛选甜菊糖苷突变体的有效方法。根据实验结果，优化的电泳条件为：60 mmol/L Tris 硼酸缓冲液(pH 8.0)，柱温30℃，工作电压25 kV。优化条件下，甜菊苷(Stevioside)迁移时间的R.S.D为0.45%(15次)，且在7.45×10-5～1.74×10-2 mol/L的浓度范围内存在良好的线性关系(r=0.9994)，甜菊主要糖苷在5 min内均可实现分离。在优化条件下，本实验研究了低能离子注入后甜菊主要糖苷含量变化，结果令人满意。
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| 关 键 词： | 低能离子注入 毛细管区带电泳法 甜菊 甜菊苷 |
| 收稿时间： | 1999-10-28 |
| 修稿时间： | 2000-03-21 |
Analysis of the Content of Stevia Sweeteners by Capillary Zone Electrophoresis |
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| SHAO Han-JuanHU Yong-GangDING LiangSHEN Ming-ShanCHEN Mu-ZhuanZHANG Xiang-Hong.Analysis of the Content of Stevia Sweeteners by Capillary Zone Electrophoresis[J].Bulletin of Botany,2001,18(1):113-117. |
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| Authors: | SHAO Han-JuanHU Yong-GangDING LiangSHEN Ming-ShanCHEN Mu-ZhuanZHANG Xiang-Hong |
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| Institution: | １(The Key Laboratory of the Minitry of Education for Cell Biology and Tumor Cell Engineering,Xiamen University, Xiamen 361005) ２(State Key Laboratory for Physical Chemical of Solid Surface, Xiamen University, Xiamen 361005) |
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| Abstract: | This paper introduced an effect capillary zone electrophoresis method for screening of stevia mutation. According to the experimental results, the optimum separation conditions were selected as: 60 mmol/L Tris tetraborate buffer of pH 8.0, 30 ℃ and 25 KV. Under optimum conditions, the R.S.D. of stevioside migration time for 15 runs was 0.45%. The detector response for stevioside was linear over the range of 7.45×10-5～1.74×10-2 mol/L (r=0.9994). Stevia main sweeteners cound be separated effectively in less than five minutes. This method was applied for determining the variation of stevia main sweeteners after low_energy ions implantation. |
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| Keywords: | , |
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