甜高粱再生体系的建立

利用甜高粱成熟种子和成熟胚作为外植体, 通过愈伤组织再生途径建立了植株再生体系。结果表明, 成熟种子在添加1.38 g.L-1脯氨酸、500 mg.L-1水解酪蛋白和3.0 mg.L-1 2, 4-D的MS培养基上可以较快地诱导出生长状态良好的愈伤组织;成熟胚在添加1.38 g.L-1脯氨酸、500 mg.L-1水解酪蛋白、2.5 mg.L-1 2, 4-D和0.1 mg.L-1KT的MS培养基上也能诱导出生长良好的愈伤组织。将愈伤组织转移到MS+1 mg.L-1 IAA + 0.5 mg.L-1 6-BA培养基上诱导芽, 然后再转移到MS+3 mg.L- 1IBA培养基上生根后, 可发育成为完整的植株。

Efficient Induction of Callus and Plant Regeneration from Seeds and Mature Embryos of Sweet Sorghum

Sweet sorghum, which can grow and develop normally in non-cultivated soil due to higher tolerance to drought, salinity and nutritional deficiency, is one of the most important energy crops. Mature seeds and embryos of two sweet sorghum cultivars(Sorghum bicolor cv. Cow ley, M81-E) were used as explants, and regeneration systems of sweet sorghum plants were successfully established via callus pathway. The results showed that Murashige and Skoog (MS) medium containing 1.38 g.L-1 proline, 500 mg.L-1 hydrolyzed casein and 3.0 mg.L-1 2,4-D was a better medium for induction and growth of callus from mature seeds, and that containing 1.38 g.L-1 proline, 500 mg.L-1 hydrolyzed casein, 2.5 mg.L-1 2,4-D and 0.1 mg.L-1 KT was a better medium for mature embryos. The shoots were induced on MS medium containing 1 mg.L-1 IAA + 0.5 mg.L-1 6-BA via callus. The roots were induced and whole plants were produced after shoots were transferred to MS medium containing 3 mg.L-1 IBA.