| 苎麻体细胞胚胎发生研究初报 |
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| 引用本文： | 陈德富,陈喜文.苎麻体细胞胚胎发生研究初报[J].植物学报,1998,15(3):65-68. |
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| 作者姓名： | 陈德富 陈喜文 |
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| 作者单位： | (南开大学生物化学与分子生物学系 天津 300071） |
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| 摘 要： | 将苎麻“浏阳大叶绿”的子叶培养在附加0.55mg/L CPA、 0.05 mg/L BR 的CXW培养基上，可获得淡黄色或浅灰绿色的颗粒状愈伤组织。将愈伤组织转移至附加0.1 mg/L CPA、 0.05 mg/L NAA 、1.5 mg/L ZT的CXW培养基上继代；颗粒状结构非常明显。继续培养在添加2 mg/L Met, 3 g/L YE 的上述继代培养基上，可分化出一些相互独立的胚状体，继而发育成为胚状体幼苗。
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| 关 键 词： | 苎麻 子叶 愈伤组织 胚胎发生 |
Preliminary Study on Somatic Embryogenesis of Ramie |
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| CHEN De-Fu and CHEN Xi-Wen.Preliminary Study on Somatic Embryogenesis of Ramie[J].Bulletin of Botany,1998,15(3):65-68. |
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| Authors: | CHEN De-Fu and CHEN Xi-Wen |
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| Institution: | (Department of Biochemistry and Molecular Biology, Nankai University, Tianjin 300071) |
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| Abstract: | The light yellow or grey green granalous calli were induced from ranie cotyledons of "Liuyang Dayelu" on exw medium contatining 0.5 mg/L CPA, 0.05 mg/L BR. When subcultured on CXW medium tontaining 0.1 mg/L CPA,O.05 mg/L NAA, 1.5 mg/L ZT, the granulons structure became more prominant. After transfered to the stone medium adding 2 mg/L Met, 3 g/L YE, some somatic embryos were induced and then grew into embryo plantlets. |
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| Keywords: | , |
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