戊二醛浓度对免疫电镜胶体金标记密度影响的定量分析

 经4％多聚甲醛+0或0.5％戊二醛固定(1小时)大豆子叶细胞，大部分细胞器和蛋白体发生较大程度的变形，甚至解体。随着固定剂中戊二醛浓度升高至1.5、2.5％，细胞超微结构保存效果提高，蛋白体内反映7S蛋白质存在的胶体金颗粒密度分别为19.7和11.4个／µm2。

Quantitative Analysis of the Effect of Glutaraldehyde Concentration on the Labelling Density Immunochemical Localization of Protein - a Colloidal Gold Particles

Most organelles and protein bodies swelled to a large extent, or were ever destroyed in the soybean cotyledon cells fixed with fixatives of 4% formaldehyde + 0,0.5 % glutaraldehyde. But the glutaraldehyde fixation with 1.5, 2.5 % formaldehyde were used as a compromise between structural and antigenic preservation, resulting in the labeling density of 19.7 and 11.4/µm2 of colloidal gold particles on the surface of protein matrix in the protein bodies, respectively.