| 麻竹花药培养及再生植株的获得 |
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| 引用本文： | 乔桂荣,李海营,蒋晶,孙宗修,卓仁英.麻竹花药培养及再生植株的获得[J].植物学报,2010,45(1):88-90. |
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| 作者姓名： | 乔桂荣 李海营 蒋晶 孙宗修 卓仁英 |
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| 作者单位： | 1中国林业科学研究院亚热带林业研究所, 富阳 311400; 2中国水稻研究所, 杭州 310006 |
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| 摘 要： | 以麻竹(Dendrocalamus latiflorus Munro)花药为材料, 于M8+2 mg·L–1 NAA +0.5 mg·L–1 6-BA+15 mg·L–1 PAA+7.5 mg·L–1 STS+500 mg·L–1 CH+100 mg·L–1 proline+100 mg·L–1 glutamin+5.4% maltose+0.8% agar的诱导培养基上成功诱导出胚性愈伤组织, 在此培养基上继代可形成体胚并分化成苗, 初步建立了麻竹花药一步成苗的再生体系。
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| 关 键 词： | 花药培养 麻竹 苯乙酸 |
| 收稿时间： | 2009-01-21 |
| 修稿时间： | 2009-03-31 |
Anther Culture and Plant Regeneration of Dendrocalamus latiflorus |
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| Guirong Qiao,Haiying Li,Jing Jiang,Zongxiu Sun,Renying Zhuo.Anther Culture and Plant Regeneration of Dendrocalamus latiflorus[J].Bulletin of Botany,2010,45(1):88-90. |
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| Authors: | Guirong Qiao Haiying Li Jing Jiang Zongxiu Sun Renying Zhuo |
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| Institution: | 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China 2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China |
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| Abstract: | Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L–1 NAA, 0.5 mg·L–1 6-BA, 15 mg·L–1 phenylacetic acid (PAA), 7.5 mg·L–1 silver thiosulfate (STS), 500 mg·L–1 casein enzymatic hydrolysate (CH), 100 mg·L–1 proline, 100 mg·L–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established. |
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