用基因枪法将抗除草剂基因导入小麦栽培品种的研究

利用基因枪法将抗除草剂bar基因导入西南地区的3个小麦栽培品种，共获得7个转基因植株，转化频率在0.45%～1.2%之间，转化周期缩短至3个月左右。对抗性植株进行PCR和PCR_Southern 杂交检测，初步确定bar基因已导入小麦基因组。做转基因植株叶片对除草剂PPT的抗性试验，有4株呈抗性，3株呈部分抗性，表明bar基因已在小麦植株中得到表达。

Studies on the Transfer of Herbicide-resistance Gene into Cultivated Wheat by Biolistics

A herbicide resistance gene bar was transferred into immature embryos of three wheat cultivars by biolistics. As a result，7 transformed plants were obtained from a total 902 bombardment embryos with the transformation frequency of 0.45～1.2% and transformation time was reduced to 3 months or so. PCR and PCR_Southern blot analysis suggested that the foreign genebar had been transferred into wheat plants. The herbicide_resistance test indicated that four plants were totally resistant to PPT and the other three were partially resistant. It suggested that bar gene was expressed in wheat plants.