黑果绣球胚的离体培养

通过不同发育时期黑果绣球(Viburnum lantana)胚的离体培养，找到了胚培养发芽率最高的取材时期，在北京地区是8月下旬到9月上旬。使用的基本培养基是MS培养基。使茎段分化的培养基是MS+BA0.5+2ip5.0+zt0.5(单位mg／L下同)，培养40天后能得到大量的苗。切取带4片叶以上的苗，转接在大量元素减半的1／2MS+IBA0.2的生根培养基上，10天以后开始生根。15天后经锻炼的小植株可移栽到瓦制花盆或营养钵中，盆土基质为1:3(体积比)的沙与草炭土的混合土，置于全光苗床(自然光照下，自控间歇喷雾保持叶面湿度。)，室温18—25℃，生长10天就可以移入冷室，按一般植物管理。成活率80%以上。

Embryo Culture of Viburnum lantana in Vitro

The optimal time of embryos of Viburnum lantana for culture in vitro is the period of late Angust-early September during which the highest break rate of the embryos was found. The basic medium consists of Murashige and Skoog (MS), The Shoots were transferred for multiplication to the medium supplemented with 0.5mg/L BA, 5.0mg/L 2ip and 0.5mg/L ZT. In 6-7 weeks, shoot of 12-15mm in height were subcultured on 1/2 MS medium with addition　of 0.2mg/L IBA and roots grow out 10 days later. After 15 days, plantlets were potted in soil of sand and peat (v/v=311) and interval misted in full sun light, plantlets accustomed to the normal growing condition after a week. The　survial rate was 80%.