| 绿色荧光蛋白及其在GMOs 生态监测中的应用 |
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| 引用本文： | 沈宝成,李梅,石纪成,张木清,米湘成,魏伟.绿色荧光蛋白及其在GMOs 生态监测中的应用[J].植物学报,2007,24(2):134-140. |
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| 作者姓名： | 沈宝成 李梅 石纪成 张木清 米湘成 魏伟 |
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| 作者单位： | 1 中国科学院植物研究所, 北京 100093;2 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室, 福州 350002 |
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| 基金项目： | 国家自然科学基金,中国科学院知识创新工程项目 |
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| 摘 要： | 绿色荧光蛋白 (green fluorescent protein, GFP)是在海洋无脊椎动物水母(Aequorea victoria)中获得的一种由238个氨基酸组成的多肽。该多肽通过翻译后加工形成生色基团, 产生稳定的荧光, 而且这种荧光很容易被检测。GFP作为动、植物以及微生物基因工程研究上的一种广泛的选择标记, 具有检测灵敏度高、操作简便、不需要添加任何底物或辅助因子等优点, 更重要的是利用GFP可对GMOs进行快速、原位、实时、活体监测。本文概括介绍了GFP的特性、改造及其检测, 并从生态学角度论述了GFP在GMOs生态监测研究中的应用及其发展前景。
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| 关 键 词： | 生物安全 生态监测 适合度 基因流 绿色荧光蛋白 |
| 收稿时间： | 2006-05-29 |
| 修稿时间： | 2006-11-16 |
Green Fluorescent Protein and Its Application in Ecological Monitoring of Genetically Modified Organisms |
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| Baocheng Shen,Mei Li,Jicheng Shi,Muqing Zhang,Xiangcheng Mi,Wei Wei.Green Fluorescent Protein and Its Application in Ecological Monitoring of Genetically Modified Organisms[J].Bulletin of Botany,2007,24(2):134-140. |
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| Authors: | Baocheng Shen Mei Li Jicheng Shi Muqing Zhang Xiangcheng Mi Wei Wei |
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| Institution: | 1 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;2 Key Laboratory of Eco-Physiology and Genetic Improvement for Sugarcane under Ministry of Agriculture, Fujian Agricultural and Forestry University, Fuzhou 350002, China |
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| Abstract: | Green fluorescent protein (GFP) is a special protein of a single polypeptide chain with 238 amino acids derived from Aequorea victoria. A chromophore is formed autocatalytically on the nascent apoprotein’s backbone via posttranslational modification.
The stable light-stimulated fluorescence is specifically independent and does not require cofactors, substrates or additional gene
products. As a valid marker gene for bacteria, animal and plant genetic engineering, GFP is very sensitive for detection and is easily manipulated. What is more important, GFP can be detected in vivo, in situ, and in real time. This paper briefly introduces the
biochemical and genetic properties of GFP and its application in the research of ecological monitoring of genetically modified
organisms (GMOs). |
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| Keywords: | , |
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