云南普通野生稻遗传多样性和亲缘关系

野生稻(Oryza rufipogon)是稻属的重要组成部分, 具有许多优良性状, 是水稻遗传改良的天然基因库。本研究通过对形态学性状的观测, 及ISSR和RAPD UPGMA聚类分析, 将云南普通野生稻划分为4个类型, 即元江类型、景洪紫杆直立型、景洪绿杆直立型和景洪匍匐型。在供试材料中筛选到具有多态性的ISSR和RAPD引物各11个, ISSR引物扩增出多态带113条, 多态性条带比率(PPB)为82.26%, RAPD引物共扩增出多态性条带76条, PPB值为76.77%, 两种分子标记的分析结果呈极显著正相关(r = 0.951)。此外UPGMA聚类结果表明, 云南普通野生稻不同类型与其它地区普通野生稻之间的遗传亲缘关系差异明显。

Assessment of Genetic Diversity and Relationship of Oryza rufipogon from Yunnan

Wild rice species such as Oryza rufipogon are an important part of Oryza, which are many excellent properties and are a gene bank for rice improvement. This paper proposes that O. rufipogon from Yunnan can be classified into 4 types, namely, Yuanjiang type and purple erect-stem type, green erect-stem type and espalier-growth type from Jinghong. Types were identified by morphologic, such as anther, earring time, and seed shape, and polymorphic analysis with random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSRs), as well as clustering analysis of markers. A total of 11 ISSR primers and 11 RAPD primers showed polymorphism among the entries. For ISSR markers, 113 polymorphic bands were generated, with 82.26% proportion of polymorphic bands (PPB). For RAPD markers, 76 polymorphic bands were generated, for 76.77% PPB. Gas spectrometry results revealed a significant positive correlation of 0.951 between RAPD and ISSR markers. Clustering analysis by UPGMA showed types of O. rufipogon from Yunnan having significantly different genetic relationships with wild rice species from other areas.